Journal: Reproduction in domestic animals = Zuchthygiene

Article Title: Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos).

doi: 10.1111/rda.12931

Figure Lengend Snippet: FIGURE 3 OX2-IR in cytotypes of the alpaca testis. (a): Small cluster of Leydig cells containing different quantity of small positive granules scattered in their cytoplasm. (b, c): Round (b) and elongated (c) spermatids showed in their cytoplasm arciform or roundish positive structures which, respectively, embraced portion of the nucleus of the younger cells or were localized in the tail of the older ones. Avidin–biotin immunohistochemical technique. Bars: 20 μm

Article Snippet: Mouse anti- OxA (MAB763) and anti- OxB (MAB734) monoclonal antibodies and their synthetic peptides were obtained from R&D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; goat polyclonal anti- OX2 antibody (sc- 8074) and its blocking peptide (sc- 8074 P) from Santa Cruz Biotechnologies (Santa Cruz, CA, USA); horseradish peroxidase- conjugated rabbit anti- goat IgG (A- 5420) and bovine serum albumin (BSA) from Sigma Chemical Co. (St. Louis, MO, USA); and biotinylated goat anti- mouse (BA- 9200), rabbit anti- goat (BA- 1000) secondary antibodies and avidin–biotin complex (PK- 6105) from Vector Laboratories (Burlingame, CA, USA).

Techniques: Avidin-Biotin Assay, Immunohistochemical staining

Journal: Reproduction in domestic animals = Zuchthygiene

Article Title: Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos).

doi: 10.1111/rda.12931

Figure Lengend Snippet: FIGURE 4 OX2 expression detected by means of Western blotting analysis of testis homogenate. Lane 1: alpaca testis. Lane 2: rat brain. Molecular weight markers are expressed in kDa and reported on the right

Article Snippet: Mouse anti- OxA (MAB763) and anti- OxB (MAB734) monoclonal antibodies and their synthetic peptides were obtained from R&D Systems (Abingdon, UK) and from Tocris Bioscience (Bristol, UK), respectively; goat polyclonal anti- OX2 antibody (sc- 8074) and its blocking peptide (sc- 8074 P) from Santa Cruz Biotechnologies (Santa Cruz, CA, USA); horseradish peroxidase- conjugated rabbit anti- goat IgG (A- 5420) and bovine serum albumin (BSA) from Sigma Chemical Co. (St. Louis, MO, USA); and biotinylated goat anti- mouse (BA- 9200), rabbit anti- goat (BA- 1000) secondary antibodies and avidin–biotin complex (PK- 6105) from Vector Laboratories (Burlingame, CA, USA).

Techniques: Expressing, Western Blot, Molecular Weight

Journal: Scientific reports

Article Title: Immunogenicity of a novel tetravalent vaccine formulation with four recombinant lipidated dengue envelope protein domain IIIs in mice.

doi: 10.1038/srep30648

Figure Lengend Snippet: Figure 2. Analysis of IgG antibody subclasses in mice immunized with a tetravalent recombinant lipidated dengue envelope protein domain III. Groups of BALB/c mice were immunized subcutaneously twice with a tetravalent vaccine (10 μg of each serotype of recombinant lipidated dengue envelope protein domain III) or PBS at a 4-week interval. Serum samples were collected 8 weeks after the first immunization. IgG1, IgG2a, IgG2b, and IgG3 antibodies against D1ED III, D2ED III, D3ED III, and D4ED III were evaluated by ELISA, respectively. Data represent the mean ± standard deviation from total 12 mice of 2 experiments.

Article Snippet: Bound IgG, IgG1, IgG2a, IgG2b, or IgG3 were detected with HRP-conjugated goat anti-mouse IgG Fc (ICN Cappel), biotin-conjugated rabbit anti-mouse IgG1 (Thermo Fisher Scientific Inc., USA), IgG2a (Thermo Fisher Scientific Inc., USA), IgG2b (Thermo Fisher Scientific Inc., USA), or IgG3 (Fitzgerald Industries International Inc.), respectively.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Scientific reports

Article Title: Immunogenicity of a novel tetravalent vaccine formulation with four recombinant lipidated dengue envelope protein domain IIIs in mice.

doi: 10.1038/srep30648

Figure Lengend Snippet: Figure 1. Induction of durable antibody responses in mice immunized with a tetravalent recombinant lipidated dengue envelope protein domain III. Groups of BALB/c mice were immunized subcutaneously twice with a tetravalent vaccine (10 μg of each serotype of recombinant lipidated dengue envelope protein domain III) or PBS at a 4-week interval. Serum samples were collected at 4, 8, 12, and 20 weeks after the first immunization. IgG antibodies against D1ED III, D2ED III, D3ED III, and D4ED III were evaluated by ELISA, respectively. Data represent the mean ± standard deviation from total 12 mice of 2 experiments.

Article Snippet: Bound IgG, IgG1, IgG2a, IgG2b, or IgG3 were detected with HRP-conjugated goat anti-mouse IgG Fc (ICN Cappel), biotin-conjugated rabbit anti-mouse IgG1 (Thermo Fisher Scientific Inc., USA), IgG2a (Thermo Fisher Scientific Inc., USA), IgG2b (Thermo Fisher Scientific Inc., USA), or IgG3 (Fitzgerald Industries International Inc.), respectively.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Scientific reports

Article Title: Immunogenicity of a novel tetravalent vaccine formulation with four recombinant lipidated dengue envelope protein domain IIIs in mice.

doi: 10.1038/srep30648

Figure Lengend Snippet: Figure 4. Analysis of IgG antibody avidity in mice immunized monovalent or tetravalent recombinant lipidated dengue envelope protein domain III. Groups of BALB/c mice were immunized subcutaneously twice with a monovalent (10 μg recombinant lipidated dengue envelope protein domain III) or a tetravalent (10 μg of each serotype of recombinant lipidated dengue envelope protein domain III) vaccine at a 4-week interval. Serum samples were collected at 8 weeks after the first immunization. Antibody avidity profiles were examined by ELISA. Data represent the mean ± standard deviation from total 10–12 mice of 2 experiments. Statistical significance was determined by Kruskal-Wallis test with Dunn’s multiple comparison test. *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: Bound IgG, IgG1, IgG2a, IgG2b, or IgG3 were detected with HRP-conjugated goat anti-mouse IgG Fc (ICN Cappel), biotin-conjugated rabbit anti-mouse IgG1 (Thermo Fisher Scientific Inc., USA), IgG2a (Thermo Fisher Scientific Inc., USA), IgG2b (Thermo Fisher Scientific Inc., USA), or IgG3 (Fitzgerald Industries International Inc.), respectively.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Standard Deviation, Comparison

Journal: PLoS ONE

Article Title: Mizoribine Ameliorates Renal Injury and Hypertension along with the Attenuation of Renal Caspase-1 Expression in Aldosterone-Salt-Treated Rats

doi: 10.1371/journal.pone.0093513

Figure Lengend Snippet: Aldosterone (ALD) enhanced the renal expression of the nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing (NLRP) 3 and caspase-1, and increased the number of TUNEL-positive cells—the hallmark of pyroptosis—whereas the increase in these factors was significantly suppressed by mizoribine (MZR). (A) Western blot analysis for NLRP3; caspase-1; and α-tubulin was performed. The total cell lysate was prepared from the rat kidney cortex. Graphs show the expression level quantified by densitometry and normalized with α-tubulin. (B) Representative photomicrographs of TUNEL staining of kidney sections. The graph shows the quantification of TUNEL-positive cells. Values are presented as mean ± SEM. * P <0.05.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti-caspase-1 antibody (Millipore, Temecula, CA, USA); rabbit polyclonal anti-nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain-containing 3 (NLRP3) antibody (Novus Biologicals, Littleton, CO, USA); mouse monoclonal anti-α-tubulin antibody (Sigma); rabbit polyclonal anti-αENaC antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA); rabbit polyclonal anti-serum-glucocorticoid regulated kinase (SGK) antibody (Cell Signaling Technology, Beverly, MA, USA); and rabbit polyclonal anti-with-no-lysine 4 (WNK4) antibody (Millipore).

Techniques: Expressing, Binding Assay, TUNEL Assay, Western Blot, Staining